A Doll s House Dramatic Irony - 1122 Words

A Doll’s House → Dramatic Irony In A Doll’s House by Henrik Ibsen, dramatic irony is displayed by providing the audience with eventful information to create a focus on the emotions of the characters. This spotlight allows for us to explore and note symbols and subtle gestures which contribute to the greater themes of appearance versus reality and sacrificial roles of women. The effective use of dramatic irony is encapsulated by the belittling of women’s roles in society during the Victorian era through his expression of his recurring doll image. The doll image can be seen vividly through an unanticipated disdain Nora builds for her unjust lifestyle, making evident that there is a gap between the doll appearance of Nora and her life and†¦show more content†¦The effect of dramatic irony here builds through Nora’s physical actions and reactions to these comments; ‘Withdraws hand’, ‘Pale with terror’. These subtle actions are observed and analyzed by the audience subconsciously. If Torvald had learned about Nora’s dishonesty, Nora would have stayed on terms of guilt rather than leave with her head held high and in the act of independence. Dramatic irony is also developed through Dr. Rank’s last goodbye to the Helmer family. In this play, Dr. Rank appears to be the only one who believes that Nora goes beyond her woman role; he treats her even above her husband, which is absurd in the Victorian Era. In this scene, Dr. Rank speaks with Nora in â€Å"code†, allowing for them to communicate clearly to one another, but saving Torvald from the tragic news. Nora and Dr. Rank speak to one another, using the masquerade as means of speaking of Dr. Rank’s fast approaching death. While speaking to Torvald and Nora, Dr. Rank replies with â€Å"At the next masquerade I’m going to be invisible [...] then no one on earth can see [me].† in which Torvald dismisses this, reasoning that â€Å"[Dr. Rank has] been drinking heavily.† (p10101011). By leaving Torvald out of this final farewell, Torvald avoids extreme feelings of sadness, allowing for Dr. Rank’s and his final interaction to be no ble andShow MoreRelatedA Doll s House By Henrik Ibsen1288 Words   |  6 Pages Henrik Ibsen’s A Doll’s House is based in the Victorian society of the 19th century. It assesses the many struggles and hardships that women faced because of marriage â€Å"laws† that were crucial during that time period. The society was male- dominated with no equality. Nora is the protagonist in A Doll’s House and the wife of a man named Torvald. This play is about Nora’s voyage to recognizing her self- determination and independence. She transforms from a traditional, reserved woman to a new, independentRead MoreHenrik Ibsen s A Doll s House1291 Words   |  6 Pages002322- 3 Henrik Ibsen’s A Doll’s House is based in the Victorian society of the 19th century. It assesses the many struggles and hardships that women faced because of marriage â€Å"laws† that were crucial during that time period. The society was male- dominated with no equality. Nora is the protagonist in A Doll’s House and the wife of a man named Torvald. This play is about Nora’s voyage to recognizing her self- determination and independence. She transforms from a traditional, reserved woman toRead MoreA Doll s House By Henrik Ibsen1288 Words   |  6 Pages Henrik Ibsen’s A Doll’s House is based in the Victorian society of the 19th century. It assesses the many struggles and hardships that women faced because of marriage â€Å"laws† that were crucial during that time period. The society was male- dominated with no equality. Nora is the protagonist in A Doll’s House and the wife of a man named Torvald. This play is about Nora’s voyage to recognizing her self- determination and independence. She transforms from a traditional, reserved woman to a new, independentRead More Comparing A Dolls House and Oedipus Rex Essay1672 Words   |  7 PagesComparing A Dolls House and Oedipus Rex Ibsens drama A Dolls House, serves as an example of the kind of issue-based drama that distinguishes Ibsen from many of his contemporaries. The plays dialogue is not poetic, but very naturalistic, and the characters are recognizable people. Given the sense of modernity which the play possesses it seems unusual to compare it to a Greek tragedy produced more than two-thousand years previously. On closer examination however, thereRead MoreHenrik Ibsen s A Doll s House1231 Words   |  5 Pages A Doll’s House is a play by Henrik Ibsen about the liberation of the protagonist, Nora, from a toxic and oppressive relationship in the Victorian Era. Based on a real friend of Ibsen, Nora portrays a seemingly childish and bubbly persona, caged by noble sacrifices and a web of innocent lies. Manipulative and careful, she works furtively to solve all of her problems independently. This contrasts the view her husband has of her as his little doll. He suppresses her freedom of speech, thought, andRead MoreHerik Ibsen: Father of Modern Drama1459 Words   |  6 Pagespioneer theatre dramaturges that began the Modernism Movement, primarily known as the Realism Movement. Modernism/Realism was a revolutionary idea back in Ibsen s time. Many concepts of theater - including plots, dialogue, and characters – were renovated in order to make theater more useful to society’s goals back then. During 1859 to 1900 s, before the rise of Realism, theatre was mainly composed of melodramas, spectacle plays, comic operas, and vaudevilles. The stories displayed did have moralRead MoreConventions of Drama1980 Words   |  8 Pagesselected from Greek, Elizabethan, Restoration and Modern times can be analysed to show and represent the changes of drama. These plays are #8220;Oedipus the King#8221;, #8220;Macbeth#8221;, #8220;The Way of the World#8221; and #8220;A Doll#8217;s House#8221;. The early origins of drama came from the Greek. Drama in Greece,450BC was not readily available to the society. Plays were only put on twice a year during great religious celebrations. At these festivals, where the plays were performedRead MoreA Dolls House As A Tragic Hero Analysis967 Words   |  4 Pagesgreatness but who through a weakness, or tragic flaw in his character, falls into the depths of misery and often to his death† (Ingham 1). Within Oedipus Rex, Sophocles laid the foundation for what is now considered the ideal tragic hero. Within A Doll’s House, Ibsen creates a modern hero in Nora Helmer; a woman who was oppressed for going against social rules for saving her husband. Nora follows the Aristotelian journey of a tragic hero, from hamartia through her tragic fall into catharsis. She is consideredRead More Conventions Of Drama Essay1926 Words   |  8 Pagesselected from Greek, Elizabethan, Restoration and Modern times can be analysed to show and represe nt the changes of drama. These plays are amp;#8220;Oedipus the King;, amp;#8220;Macbeth;, amp;#8220;The Way of the World; and amp;#8220;A Dollamp;#8217;s House;. The early origins of drama came from the Greek. Drama in Greece,450BC was not readily available to the society. Plays were only put on twice a year during great religious celebrations. At these festivals, where the plays were performedRead MoreTo Kill a Mockingbird1617 Words   |  7 Pagesâ€Å"Boo† Radley by his domineering father: Inside the house lived a malevolent phantom. People said he existed, but Jem and I had never seen him. People said he went out at night when the moon was down, and peeped in windows. When people s azaleas froze in a cold snap, it was because he had breathed on them. Any stealthy small crimes committed in Maycomb were his work. Once the town was terrorized by a series of morbid nocturnal events: people s chickens and household pets were found mutilated; although

Coraline free essay sample

In response to the school board’s request for student feedback, I am writing to express my opinion about which should be used to teach about the Fantasy genre, Coraline the book or Coraline the movie. In my opinion, I believe that Coraline the movie should be used in schools to teach the Fantasy genre. This is because the sounds, animations, and new characters all show more elements and examples of the Fantasy genre than in the book. By the time you finish reading this, you will surely end up being persuaded that Coraline the movie has more elements and examples of Fantasy than in the book. The first detail that makes Coraline the movie have more elements of Fantasy is animation. The animations in the movie were cartoon people, which means the characters don’t necessarily have to do things real people can do. Another element of animation that makes the movie more fantastical is that you can see exactly what the character is doing but in the book it is just your imagination. We will write a custom essay sample on Coraline or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page Also, in the movie, special effects can be added to make the scenes look more fantastical. Overall, the animations from the movie show more elements of fantasy than in the book. Secondly, the new and different characters in the movie make Coraline the movie have more elements of fantasy than the book. In the movie, the â€Å"other mother† turns the â€Å"other dad† into a pumpkin instead of a blob. This is more fantastical because pumpkins are plants and the fact that a pumpkin is alive and speaks is very fantastical. The second example of new and different characters is Wybie Lovat. He is a new character that is in the movie but not the book. He adds more fantasy because he finds a doll that looks just like Coraline and gives it to her. The new and different characters is the second detail that makes Coraline the movie have more elements of Fantasy. The third detail that makes Coraline the movie have more elements of Fantasy is sound. The sound from the movie can let you hear the characters tone of voice while speaking which lets you know if what they are saying sounds fantastical. The second thing is that you can hear background sounds and noise. This adds to Fantasy because it lets you hear strange sounds in the background that make it more fantastical. The third thing is that in the movie you can hear how loud the character is speaking. This can let you know how the character feels which adds on to the fantastical elements. I have now expressed my opinion on which to use to teach the Fantasy genre. Coraline the movie has many elements and examples of Fantasy. Coraline the movie should be used in schools to teach the Fantasy genre because the sounds, animations, and new or different characters all show more elements and examples of the Fantasy genre than in the book. Now that you have taken a look at many examples and elements of Fantasy in the movie, I hope that you consider using Coraline the movie to teach Fantasy in schools.

Naadac Code of Ethics free essay sample

After reading the NAADAC Code of Ethics I have come to realize how any violation of them could impact either a client or the Human Services Profession as a whole. Any profession, such as the Human Services, that has the capacity to do so much good for an individual also has the same capacity to do them harm. In order to prevent such harm a code of ethics must be created and followed by everyone in that profession. The NAADAC Code of Ethics consists of nine principles. These principles, though few in number, are powerful in content. They help guide and maintain a helpful relationship with our clients and prevent a hurtful one. Some of the challenges I foresee having with NAADAC’s Code of Ethics will be easier to deal with than others. Principle 2 talks about client welfare. It states, â€Å"I shall not do for others what they can readily do for themselves but rather, facilitate and support the doing. We will write a custom essay sample on Naadac Code of Ethics or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page † In the past I have often found myself â€Å"helping† others in a way that I will have to watch out for. I have a tendency to catch myself doing for others and not realizing that that’s not helping but doing. I understand the basis behind letting the clients do for themselves. A sense of accomplishment and control are necessary to a clients well being. Doing for a client what they can do for themselves is not helpful at all. Dual Relationships, as described in Principle 7, seems to me to be the biggest issue a helping professional is faced with on a continuous basis. Although I feel that I will not have a huge problem in this area, I know that this issue requires constant and diligent awareness. In order to maintain a professional and helping relationship with a client certain lines must be drawn. Even though we are human and imperfect, we must maintain a professional relationship with our clients and never exploit the obvious power differential that exists between us for personal gain. To do so would undermine the entire profession, our agencies, and the relationships we have with our clients. The only other principle I see that may be an issue for some people is number 8. Principle 8 covers the reporting of individuals that may be an actual or likely source of harm to clients, colleagues, institutions, or the profession. Personally, I can understand why this would be a difficult subject. Reporting someone to the authorities for the sake of preventing harm to themselves or others, however difficult, is the right thing to do. Some would call it â€Å"narking†, â€Å"snitching†, or â€Å"ratting† someone out, but in the helping profession to do otherwise would be a failure on our part to do just that†¦. help. I chose this field of study to help others the way that I have been helped and to give back to the world what has so freely been given to me and I gladly accept NAADAC’s Code of Ethics. When we enter this profession we are given great power and with great power comes great responsibility.

Your Topic Is Pharmacogenomics Example

Your Topic Is Pharmacogenomics Example Your Topic Is Pharmacogenomics – Coursework Example Pharmacogenomics This paper contains the basic definition of Pharmacogenomics and the beneficial and harmful effects imposed by this biotechnology will also be discussed. Pharmacogenomics; its Beneficial and Harmful Effects The term Pharmacogenomics is a combination of pharmacology and genomics and it is the study which explains how different people respond to medicines and other drugs depending on their genetic composition. It is actually the gene factor in each individual which entails the difference in responses. Apart from genetic framework, there are a number of reasons that contribute to the variation in attitudes, they can be diet, environment, lifestyle, age factor etc but the key to manufacturing tailor made medicines totally depends on how well the doctors understand the genetic makeup of individuals. (Martin, 11) Beneficial EffectsPharmacogenomics makes it easy for individuals to identify their respective gene code which in turn enables them to maintain a lifestyle which i s in accordance to it so that the genetic complexities which might prove to be harmful in the future can be avoided. Moreover disease vulnerability can be detected at an early age and the appropriate treatments and therapies can be offered at the right stage. Initially medicines were diagnosed considering the patient’s age and BMI; however with the advent of Pharmacogenomics, the parameters for diagnosis have changed. Doctors now prescribe medicines keeping in view the genetics, body response to medicine etc which also decreases the chances of overdose. Harmful Effects Information related to pharmacogenomics can lead to psychological disorder in some individuals since they find it depressing to know that they might be subjected to some genetic disorder in the future. Awareness of predisposition to diseases can lead to depressing state of some people. One of the social issues that have been observed due to pharmacogenomics is that it widens the gap between the rich and the po or since the information is available mostly to the rich people since they are more concerned about their health issues whereas poor people on the other hand are least bothered and are usually not aware of this technology due to which a discrimination line is automatically drawn between the two. (Mark, 115) Mark, Rothstein. Pharmacogenomics: Social, Ethical and Clinical Dimensions. 2003. Martin, Zdanowicz. Concepts in Pharmacogenomics. 2010.

Compensation, Rewards, and Incentives Research Paper

Compensation, Rewards, and Incentives - Research Paper Example The various compensation, rewards and incentives programs designed By IBM have facilitated the organization to draw quality and skilled workforce and maintain workplace satisfaction of the existing employees. Furthermore, the monetary and non-monetary incentive programs practiced by IBM have facilitated the organization to prevent competent and quality employees from leaving the organization along with motivating them to perform dedicatedly in the workplace. The benefit programs have enabled the organization to foster and deliver enhanced employee development. Furthermore, the benefit programs of IBM have facilitated the organization to profitably respond to the rapidly altering market and industry trends. The benefit programs have also facilitated IBM to maintain skilled and agile workforce within the organization. The increased level of employee satisfaction within the organization has enabled IBM to quickly and effectively meet the supply and demand scenario as well as positively manage and develop costs and other revenue generation prospects associated with its performance (Boudreau, 2010). These factors have had a deep-rooted impact on IBM’s attainment of being on the ‘Best Places to Work For’

Computer-aided image processing for bacterial cell enumeration Essay

Computer-aided image processing for bacterial cell enumeration - Essay Example There appear several inhibitors in the way of counting colonies to ensure effectiveness of the method. The inhibitors include; methods of handling confluent development of the development of colonies that lean or overlaps other colonies, the methods of differentiating colonies regardless of the different form, volume, their feel, light intensity amongst other factors (Daims, 2007) The methods ensure accuracy in counting. A devise termed as a colony counter assists in including colonies in bacteria and varied microorganisms thriving in an algae plate. The method applied in overcoming the hindrances includes thresholding, segmentation methods, time domain frequency method, watersheding method, edge detection and morphology operator methods, regional descriptors amongst others. These methods emit a heightened level of accurateness. The projected counter proves highly reliable at both levels of correctness and memory; it also proves flexible and efficient in terms of effort and time savi ng (National Renewable Energy Laboratory (U.S.) and United States, 2006) Keywords Some of the key words in the study includes Bacteria colony, thresholding, morphology, water shedding. Introduction Bacteria colony counting appears vital in quantitative, accurate judgment of pathogens in clinical investigations and diagnosis. The manual process of counting bacteria appears challenging. The use of an automated colony counting helps in saving time and reduces labor requirements. In a bid to create fast and accurate information, and cut down the use of labor workload, two colony counting techniques come in play. The techniques might apply the use of direct and indirect process of counting the colonies. The tradition plate count technique pauses as an indirect technique and often preferred for its cheap nature. Technologies applied for quantification of microbial growth involves: ATP Bioluminescence, spiral plating, the use of membrane filtration, Direct Epi-Florescent Filter Microscopy and Membrane laser scanning, Fluorescence amongst others. Various hindrances require addressing for the techniques that count the present colonies. The techniques supply a heightened degree of correctness in counting that could be attained by applying the use of a trained hand on operator. The machine technique; allows the Bacteria to grow on a filter within a period of 24to 48 hours to examine the contamination level of the sample (Mittal and Matsko, 2012).   The microbiologists’ uses dye to identify the bacteria clearly and ease the counting process. In an industry setting, thousands of such samples appear per day; the colonies on each side remain counted manually. This consumes a lot of time and increases the chances of errors. The aim of the project remains to create software that saves time and produces the exact outcomes and ensures competence to customers. The project extension designs allows for colonies counting for a period of 6 to 8 hours before, which saves time . The paper seems to differentiate the processing of colony figures with colored data; the filter method applies to deionize (Masters, 2006).   Bacteria Bacteria pause as nuclear microorganisms; that thrives in various surfaces of the earth. Bacteriology, the study of bacteria, is a branch in micro biology. Bacteria can grow anywhere on the face of the earth, in soil, acid hot springs amongst other places. Grouped bacteria originate from one common bacterium. A bacteria cell

Polycyclic Aromatic Hydrocarbons (PAHs)

Polycyclic Aromatic Hydrocarbons (PAHs) Introduction Polycyclic aromatic hydrocarbons (PAHs) are made of two or more fused aromatic rings, where PAHs with two or three rings have low molecular weight and acute toxicity whereas PAHs with high molecular weight (4 or more rings) have shown high carcinogenic potential. These compounds are often products of incomplete combustion reactions where there is a lack of oxygen, there are two types of PAH; natural and anthropogenic which can be found in many different kinds of samples . However, the ratio of natural processes to anthropogenic processes is very low. PAHs can form due to a variety of anthropogenic sources the main one is believed to be atmospheric decomposition, other sources include thermal combustion processes, vehicular emissions and biomass burning [3]. PAHs caused from incomplete combustion reactions reach the sea via the atmosphere and rivers . Due to the causes listed above the United States Environmental Protection Agency (EPA) and other agencies formed regulations for the detection and monitoring of PAHs in the human environment, with new analytical methods being produced with better sensitivity and selectivity . Overall the EPA has selected sixteen PAHs as priority pollutants [4]. To verify different PAHs in seawater is difficult as the overall solubility of PAHs in water is low, and continues to decrease as molecular weight increases, the low solubility in turn effects the hydrophobic nature (between 3 and 8) and therefore the concentration of PAHs dissolved in seawater is very low . There are a variety of methods used to analyse the PAHs in seawater and other types of water. High Performance Liquid Chromatography (HPLC) is the most widely used analytical technique in analysing PAHs in seawater samples; in this document HPLC will be discussed along with other analytical methods such as Gas Chromatograph-Mass Spectrometer (GC-MS). This document focuses on data and results from the following papers: â€Å"Determination of polycyclic aromatic hydrocarbons in seawater by high-performance liquid chromatography with fluorescence detection following micelle-mediated preconcentration† V. Pino et al. â€Å"Polycyclic Aromatic Hydrocarbons in Water and Sediment of the Baltic Sea† â€Å"GC-MS Analysis of Total Petroleum Hydrocarbons and Polycyclic Aromatic Hydrocarbons in Seawater Samples after the North CapeOil Spill† Christopher M. Reddy and James G. Quinn Each paper will be individually introduced and the sample preparation, analytical method and any results significant to the separation will be discussed. The methods will then be compared, giving the advantages and disadvantages of each technique. â€Å"Determination of polycyclic aromatic hydrocarbons in seawater by high-performance liquid chromatography with fluorescence detection following micelle-mediated preconcentration† V. Pino et al. This paper looked at how non-ionic surfactant polyoxyethylene-10-lauryl ether (POLE) can be used for extraction of PAHs in seawater and compared cloud-point extraction (CPE)-HPLC and liquid-liquid extraction (LLE)-GC-MS. Sample Preparation The sample was prepared by shaking fuel-oil with natural seawater for one hour, and then allowed to settle and age overnight, until phase separation. The aqueous layer was removed and mixed with POLE until a final solution of 1% (w/v) was reached in the surfactant. Following this 3.0 ml of aliquots in the presence of the 1% surfactant solution dissolved in artificial seawater were administered to CPE. In order to test the reliability of the method used for preconcentration and separation of PAHs, the PAHs were analysed from spiked and un-spiked seawater samples. The method was evaluated by using LLE and GC-MS, overall determination was processed using the mass values which related to the molecular ions of the different PAHs . Analysis Once the two phases had been separated, 25 ÃŽ ¼l of the surfactant-rich phase was injected into the system. The HPLC method used in this instance to determine and separate the PAHs consisted of gradient elution with a fluorescence detector. The mobile phase was a mixture of acetonitrile and water with a linear gradient from 55 to 100% acetonitrile for 30 minutes and 100% acetonitrile for 10 minutes. The column used for the analysis was a reversed phase C18 column (Table 1) with a ÃŽ ¼Bondapak C18 guard column. Table 1: Vydac 201TP54 Reversed Phase C18Column Column length x internal diameter / mm 250 x 4.6 Particle size / ÃŽ ¼m 5 Pore size / Ã… 300 A flow rate of 1 ml / minute was used to elute the compounds. Results Each PAH gave an acceptable retention time with a relative standard deviation between 0.64 and 0.83%. The seawater contaminated with fuel-oil were analysed by both CPE-HPLC and LLE-GC-MS (Table 3). As can be seen the PAHs with fewer aromatic rings have similar results with both techniques where as the PAHs with more aromatic rings are different this could be due to the lower sensitivity in GC-MS. â€Å"Polycyclic Aromatic Hydrocarbons in Water and Sediment of the Baltic Sea† G.Witt During the years 1992 to 1994, 15 PAHs were analysed in seawater from the Baltic Sea during the different seasons. The method used is based on HPLC with fluorescence detection. Sample Preparation 156 seawater samples were collected from various depths, surface water (2-15m), bottom water (below the halocline) and surface micro layer (0-0.2 cm). The PAHs from the samples were separated and extracted via a HPLC column; three fractions were obtained using 9 ml pentane, 9 ml pentane/10% dichloromethane and 9 ml acetone as the eluents. The PAHs were contained into the second fraction and was evaporated down to 500 ÃŽ ¼l. Analysis The HPLC column used to separate the sample in to fractions was a MERCK LiChrospher Si 100-5 (Table 4) Table 4: MERCK LiChrospher Si 100-5 Column length x internal diameter / mm 250 x 4 Particle size / ÃŽ ¼l 5 Pore diameter / Ã… 60 Matrix Silica gel The column used in the analysis of the PAHs was a GROM PAH using gradient elution (Table 5). Table 5: GROM PAH Column length x internal diameter / mm 250 x 4 Eluents A) Water B) CAN Flow rate / ml min-1 1 Temperature /  °C 20 A fluorescence detector was used, along with a wavelength programme for optimal detection of all PAHs (Table 6). From the 16 PAHs listed as priority pollutants, acenaphthylene was the only one not detected as it does not show fluorescence activity. Results It was seen that there were a greater amount of lower molecular weight PAHs varying in concentration from 1 to 4.5 ng/L-1 in relation to higher molecular weight PAHs which remained below ng/L-1. The concentration of PAHs in different seasons was also compared, it was seen that autumn had the highest concentration this maybe because of low microbial degradation and photo-oxidation during the season; and summer had the lowest. During the winter months, the temperature of seawater is low which inhibits the microbial degradation of the PAHs. In spring the plankton are in abundance and thus cause a large amount of loose matter to be produced which may transfer the PAHs to the sea floor. This was mostly true for two/three ring PAHs, four to six rings however have a higher persistence to microbial degradation. The amount of PAHs in the surface micro layer was 2-13 times higher than that of the water below; this can be due to the atmospheric input . â€Å"GC-MS Analysis of Total Petroleum Hydrocarbons and Polycyclic Aromatic Hydrocarbons in Seawater Samples after the North CapeOil Spill† Christopher M. Reddy and James G. Quinn [6] GC-MS was used to measure PAHs in seawater samples after the North Cape oil spill. Sample Preparation The samples were spiked with internal standards which consisted of the following: 10 to 25 ÃŽ ¼g docosane and 2 ÃŽ ¼g each of naphthalene-d8, biphenyl-d10, acenaphthene-d10, anthracene-d10 and perylene-d12. After the samples were spiked they were shaken and extracted once with 100 ml methylene chloride and twice more with 100 ml of hexane. The organic extracts were then combined and rotary-evaporated to 1 ml while being solvent-exchanged into hexane. Following this the hexane extract was chromatographed and the first fraction (obtained by eluting with 20 ml of a 70/30 mixture of hexane/methylene chloride) containing the aromatic hydrocarbons was rotary-evaporated to a small volume (100 ÃŽ ¼l) and spiked with an external recovery standard (1.2 ÃŽ ¼g o-terphenyl in isooctane), and injected into the GC-MS detector. Analysis The PAHs were first extracted with methylene chloride and hexane and then fractionated via HPLC using silica gel. The analysis was done using a Hewlett-Packard 5890 Series II GC with a Hewlett-Packard 5971 mass selective detector. After the sample was injected, the compounds were separated using a 30m JW Scientific DB-XLB fused silica-capillary column (Table 7). Table 7:JW Scientific DB-XLB fused silica-capillary column Column length x internal diameter / mm 30,000 x 0.25 Film thickness / ÃŽ ¼m 0.25 Temperature limit /  °C 340-360 According to the website [7] this column has low polarity and is excellent for PAHs. The oven was set at 70 °C for 1.5 minutes, then ramped to 320 °C at 10 °C per minute, and then held for a further 10 minutes. The detector was run in selected-ion monitoring (SIM) mode for ions that have an m/z of 50 to 550, PAHs usually range between 128 and 234 m/z. Results A total of 54 seawater samples were collected and analysed were collected between day 4 and day 132 after the North Cape oil spill, results from a week after the oil spill showed that there were high concentration of PAHs, with the total sum coming too 115 ÃŽ ¼g L-1, these are some of the highest values for a marine oil spill. The relative percentage deviations for duplicate samples for total PAHs came to The use of other GC detectors such as GC flame ionisation detector which is widely used may not be suitable for the detection of PAHs as often lower limits of detection are needed, which is why GC-MS would be used. Comparison of analytical methods The most popular technique for the separation and determination of PAHs is HPLC however; a disadvantage of this is that gradient elution must be used opposed to isocratic elution due to the samples complexity. Other factors include that HPLC is expensive, and that it requires large amounts of solvent especially when gradient elution is used. The paper by V. Pino et al. and the paper by G. Witt both used HPLC reversed phase column with a fluorescence detector, however Pino et al. used a surfactant with a low retention time (POLE) which allowed for the determination of PAHs which were more polar. In Figure 2 the compounds eluted between ~7 and 40 minutes using the above method; whereas the method used by Witt produced a chromatogram (Figure 3) which shows the products eluted between ~7 and 23 minutes. The difference in retention times shows that the method used by Witt was more useful. However, the samples of seawater used for analysis by each paper were from different sources, Pino et al. does not state where the seawater samples were taken, while Witt took the sample from the Baltic Sea. Pino et al. only extracted 14 PAHs but Witt extracted all 16 PAHs listed by the EPA. From the data listed in Tables 2 and 6 it can be seen that there are comparable results such as Phenanthrene which eluted in 13.9 minutes for Pino et al. and 12.2 minutes for Witt, the emission wavelengths measured were identical at 370 nm and that the excitation wavelength only differed by 2 nm between Pino (248 nm) and Witt (246 nm). Overall, the HPLC methods used by both papers shows that it is a reliable method for determining PAHs in seawater, a fluorescence detector was used as the filters select the excitation and emission wavelengths and it reduces the complexity of the chromatogram which provides orthogonal information. Even though HPLC seems the most popular technique GC-MS is being used to analyse the PAHs after they have been separated by HPLC. This change could be because GC is faster than HPLC and that with a mass spectrometer (MS) detector it is possible to combine detection, quantification and identification. GC-MS is especially good for complex mixtures such as seawater where several analytes have similar retention times.